Epredia™ Lab Vision™ SREBP-1 (Sterol Regulatory Element Binding Protein-1) Ab-1 Mouse Monoclonal Antibody, 200μg/mL, BSA and azide

Description

• SREBP-1 and -2 are transcription factors which participate in the control of cholesterol homeostasis
• SREBPs proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol
• Upon activation of SREBP-1 or -2, an ∽480-500 amino acid, N-terminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis
• SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs
• SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes
• Host Species: Mouse Clone: 2A4 Isotype: IgG1/κ Species Reactivity: Human and Hamster
• Others not known
• Epitope: aa 301-407 Immunogen: Recombinant protein encoding N-terminal amino acids 301-407 (the bHLH/leucine zipper domain) of human SREBP-1 Molecular Weight: 125kDa (precursor) and 60-70kDa (cleaved) Positive Control: HeLa and IMR5 cells Cellular Localization: Cytoplasmic and nuclear Recommended for: Western Blotting Immunoprecipitation (Not verified) Note: USA: RUO; Int'l: RUO