NBP247786A

EMI1 Antibody (EMI1/1176) - Azide and BSA Free, Novus Biologicals™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP247786A-Each-of-1 In Stock ₹ 53,688.90

NBP247786A - Each of 1

₹ 53,688.90

In Stock

Quantity

1

Base Price: ₹ 53,688.90

GST (18%): ₹ 9,664.002

Total Price: ₹ 63,352.902

Antigen

EMI1

Classification

Monoclonal

Concentration

1.0 mg/mL

Dilution

Western Blot : 1 - 2 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias

EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Host Species

Mouse

Molecular Weight of Antigen

56 kDa

Quantity

0.1 mg

Primary or Secondary

Primary

Test Specificity

It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype

IgG2a κ

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone

EMI1/1176

Conjugate

Unconjugated

Formulation

PBS with No Preservative

Gene Symbols

FBXO5

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Purification Method

Protein A or G purified

Regulatory Status

RUO

Gene ID (Entrez)

26271

Target Species

Human

Form

Purified

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Description

  • EMI1 Monoclonal specifically detects EMI1 in Human samples
  • It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array.

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Fischer Scientific

NBP247786A

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Antigen:
EMI1

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Western Blot : 1 - 2 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Host Species:
Mouse

Molecular Weight of Antigen:
56 kDa

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
EMI1/1176

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
FBXO5

Immunogen:
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Gene ID (Entrez):
26271

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP247786B

--


Antigen:
EMI1

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Western Blot : 1 - 2 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Host Species:
Mouse

Molecular Weight of Antigen:
56 kDa

Quantity:
0.2 mg

Primary or Secondary:
Primary

Test Specificity:
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
EMI1/1176

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
FBXO5

Immunogen:
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Gene ID (Entrez):
26271

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP247788A

--


Antigen:
beta-1,3-Glucuronyltransferase 1/B3GAT1

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Immunohistochemistry-Paraffin : 2 - 4 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml

Gene Alias:
Beta-1,3-glucuronyltransferase 1, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), EC 2.4.1.135, galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, GlcAT-P, GLCATPGLCUATP, glcUAT-P, Glucuronosyltransferase P, NK1, NK-1, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgM κ

Applications:
Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
SPM527

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
B3GAT1

Immunogen:
Human peripheral blood mononuclear cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Gene ID (Entrez):
27087

Target Species:
Human, Rat (Negative)

Form:
Purified

Img

Fischer Scientific

NBP247788B

--


Antigen:
beta-1,3-Glucuronyltransferase 1/B3GAT1

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Immunohistochemistry-Paraffin : 2 - 4 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml

Gene Alias:
Beta-1,3-glucuronyltransferase 1, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), EC 2.4.1.135, galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, GlcAT-P, GLCATPGLCUATP, glcUAT-P, Glucuronosyltransferase P, NK1, NK-1, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.2 mg

Primary or Secondary:
Primary

Test Specificity:
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgM κ

Applications:
Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
SPM527

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
B3GAT1

Immunogen:
Human peripheral blood mononuclear cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Gene ID (Entrez):
27087

Target Species:
Human, Rat (Negative)

Form:
Purified