Manufacturer: Fischer Scientific
Pack Size | SKU | Availability | Price |
---|---|---|---|
Each of 1 | NBP247786A-Each-of-1 | In Stock | ₹ 53,688.90 |
NBP247786A - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,688.90
GST (18%): ₹ 9,664.002
Total Price: ₹ 63,352.902
EMI1
Monoclonal
1.0 mg/mL
Western Blot : 1 - 2 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
Mouse
56 kDa
0.1 mg
Primary
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG2a κ
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
EMI1/1176
Unconjugated
PBS with No Preservative
FBXO5
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Protein A or G purified
RUO
26271
Human
Purified