ZAP70 Antibody (2F3.2) - IHC-Prediluted, Novus Biologicals™
Manufacturer: Novus Biologicals
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP248224-Each-of-1 | In Stock | ₹ 45,860.16 |
NBP248224 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,860.16
GST (18%): ₹ 8,254.829
Total Price: ₹ 54,114.989
Antigen
ZAP70
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols
ZAP70
Immunogen
Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (Uniprot: P43403)
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Content And Storage
Store at 4C.
Isotype
IgG2a κ
Applications
Immunohistochemistry, Immunohistochemistry (Paraffin)
Clone
2F3.2
Dilution
Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias
70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
Host Species
Mouse
Molecular Weight of Antigen
70 kDa
Quantity
7 mL
Research Discipline
Adaptive Immunity, Cell Biology, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases
Gene ID (Entrez)
7535
Target Species
Human
Form
Purified
Related Products
Description
- ZAP70 Monoclonal specifically detects ZAP70 in Human samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.
Compare Similar Items
Show Difference
Antigen: ZAP70
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: ZAP70
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (Uniprot: P43403)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Content And Storage: Store at 4C.
Isotype: IgG2a κ
Applications: Immunohistochemistry, Immunohistochemistry (Paraffin)
Clone: 2F3.2
Dilution: Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias: 70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
Host Species: Mouse
Molecular Weight of Antigen: 70 kDa
Quantity: 7 mL
Research Discipline: Adaptive Immunity, Cell Biology, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases
Gene ID (Entrez): 7535
Target Species: Human
Form: Purified
Antigen: pan Cytokeratin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 10 mM PBS with 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: KRT1
Immunogen: This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Applications: Immunohistochemistry, Immunohistochemistry (Paraffin)
Clone: AE-1/AE-3
Dilution: Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias: __
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 7 mL
Research Discipline: Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
Gene ID (Entrez): 3848
Target Species: Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
Form: Purified
Antigen: ICAM-3/CD50
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: ICAM3
Immunogen: Recombinant human ICAM-3/CD50 protein (Uniprot: P32942)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Content And Storage: Store at 4C.
Isotype: IgG2b κ
Applications: Immunohistochemistry (Paraffin)
Clone: ICAM3/1019
Dilution: Immunohistochemistry-Paraffin
Gene Alias: CD50, CD50 antigen, CDW50CDw50, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 7 mL
Research Discipline: Cellular Markers, Immunology
Gene ID (Entrez): 3385
Target Species: Human
Form: Purified