OP146100UG
MilliporeSigma™ anti-MDM2 Clone: 5B10C,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | OP146100UG-Each-of-1 | In Stock | ₹ 45,123.00 |
OP146100UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,123.00
GST (18%): ₹ 8,122.14
Total Price: ₹ 53,245.14
Antigen
MDM2
Classification
Monoclonal
Conjugate
Unconjugated
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation
Clone
5B10C
Formulation
In 50mM sodium phosphate buffer, 50% glycerol, pH 7.5.
Immunogen
recombinant human MDM2
Regulatory Status
RUO
Test Specificity
Recognizes the ∽90kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.
Content And Storage
−20°C
Isotype
IgG2a
Related Products
Description
- Specifically detects MDM2 Clone: 5B10C in Human samples, and it is validated for Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation Increased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events
- Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells
- The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas
- Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation
- MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators
- MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators
- A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo
- Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery
- Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene
- MDM2 amplification has been observed in sarcomas and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors
- Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway
- Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function
Compare Similar Items
Show Difference
Antigen: MDM2
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation
Clone: 5B10C
Formulation: In 50mM sodium phosphate buffer, 50% glycerol, pH 7.5.
Immunogen: recombinant human MDM2
Regulatory Status: RUO
Test Specificity: Recognizes the ∽90kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.
Content And Storage: −20°C
Isotype: IgG2a
Antigen:
MDM2
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation
Clone:
5B10C
Formulation:
In 50mM sodium phosphate buffer, 50% glycerol, pH 7.5.
Immunogen:
recombinant human MDM2
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽90kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.
Content And Storage:
−20°C
Isotype:
IgG2a
Antigen: c-ErbB2/c-Neu
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone: 9G6
Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen: cells overexpressing human c-ErbB2/c-Neu
Regulatory Status: RUO
Test Specificity: Recognizes the ∽185kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
Content And Storage: 2°C to 8°C
Isotype: IgG1
Antigen:
c-ErbB2/c-Neu
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone:
9G6
Formulation:
In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen:
cells overexpressing human c-ErbB2/c-Neu
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽185kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
Content And Storage:
2°C to 8°C
Isotype:
IgG1
Antigen: c-ErbB2/c-Neu
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Form: Liquid
Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Clone: 3B5
Formulation: In 0.05M sodium phosphate buffer, 0.2% gelatin, ≤0.1% Sodium Azide.
Immunogen: a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
Regulatory Status: RUO
Test Specificity: __
Content And Storage: 2°-8°C
Isotype: IgG1
Antigen:
c-ErbB2/c-Neu
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Liquid
Applications:
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Clone:
3B5
Formulation:
In 0.05M sodium phosphate buffer, 0.2% gelatin, ≤0.1% Sodium Azide.
Immunogen:
a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
Regulatory Status:
RUO
Test Specificity:
__
Content And Storage:
2°-8°C
Isotype:
IgG1
Antigen: c-ErbB2/c-Neu
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human, Mouse
Form: Purified
Applications: Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone: 3B5
Formulation: In 0.05M sodium phosphate buffer, 0.2% gelatin.
Immunogen: a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
Regulatory Status: RUO
Test Specificity: Recognizes the ∽185kDa c-ErbB2/c-Neu protein in HEK293 cells.
Content And Storage: 2°C to 8°C
Isotype: IgG1
Antigen:
c-ErbB2/c-Neu
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Form:
Purified
Applications:
Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone:
3B5
Formulation:
In 0.05M sodium phosphate buffer, 0.2% gelatin.
Immunogen:
a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽185kDa c-ErbB2/c-Neu protein in HEK293 cells.
Content And Storage:
2°C to 8°C
Isotype:
IgG1