OP46100UG
MilliporeSigma™ anti-MDM2 Clone: IF2,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | OP46100UG-Each-of-1 | In Stock | ₹ 47,961.51 |
OP46100UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,961.51
GST (18%): ₹ 8,633.072
Total Price: ₹ 56,594.582
Antigen
MDM2
Classification
Monoclonal
Conjugate
Unconjugated
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone
IF2
Formulation
In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen
human MDM2
Regulatory Status
RUO
Test Specificity
Recognizes the ∽90kDa (apparent MW) MDM2 protein. Also recognizes isoforms of ∽57kDa and ∽74/76kDa by immunoblotting.
Content And Storage
2°C to 8°C
Isotype
IgG2b
Related Products
Description
- Specifically detects MDM2 Clone: IF2 in Human samples, and it is validated for Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting Increased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events
- Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells
- The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas
- Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation
- MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators
- MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators
- A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo
- Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes, and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery
- Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene
- MDM2 amplification has been observed in sarcomas, and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors
- Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway
- Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function.
Compare Similar Items
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Antigen: MDM2
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone: IF2
Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen: human MDM2
Regulatory Status: RUO
Test Specificity: Recognizes the ∽90kDa (apparent MW) MDM2 protein. Also recognizes isoforms of ∽57kDa and ∽74/76kDa by immunoblotting.
Content And Storage: 2°C to 8°C
Isotype: IgG2b
Antigen:
MDM2
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone:
IF2
Formulation:
In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen:
human MDM2
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽90kDa (apparent MW) MDM2 protein. Also recognizes isoforms of ∽57kDa and ∽74/76kDa by immunoblotting.
Content And Storage:
2°C to 8°C
Isotype:
IgG2b
Antigen: c-ErbB2/c-Neu
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunoblot
Clone: OD-3
Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen: cells overexpressing human c-Neu
Regulatory Status: RUO
Test Specificity: Recognizes the ∽97-115kDa truncated form of c-ErbB2/c-Neu in human serum as well as the ∽185kDa full-length protein. Does not cross-react with the EGF receptor.
Content And Storage: 2°C to 8°C
Isotype: IgM
Antigen:
c-ErbB2/c-Neu
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunoblot
Clone:
OD-3
Formulation:
In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen:
cells overexpressing human c-Neu
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽97-115kDa truncated form of c-ErbB2/c-Neu in human serum as well as the ∽185kDa full-length protein. Does not cross-react with the EGF receptor.
Content And Storage:
2°C to 8°C
Isotype:
IgM
Antigen: APC
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Form: Purified
Applications: Immunocytochemistry, Immunofluorescence
Clone: CC-1
Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen: a recombinant protein consisting of amino acids 1-226 of APC
Regulatory Status: RUO
Test Specificity: Recognizes APC in oligodendrocytes and astrocytes. Antibody Target Gene Symbol: APC Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC Entrez Gene Name: adenomatous polyposis coli
Content And Storage: 2°C to 8°C
Isotype: IgG2b
Antigen:
APC
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Form:
Purified
Applications:
Immunocytochemistry, Immunofluorescence
Clone:
CC-1
Formulation:
In 50mM sodium phosphate buffer, 0.2% gelatin.
Immunogen:
a recombinant protein consisting of amino acids 1-226 of APC
Regulatory Status:
RUO
Test Specificity:
Recognizes APC in oligodendrocytes and astrocytes. Antibody Target Gene Symbol: APC Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC Entrez Gene Name: adenomatous polyposis coli
Content And Storage:
2°C to 8°C
Isotype:
IgG2b
Antigen: BRCA1
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone: MS110
Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Immunogen: recombinant human BRCA1
Regulatory Status: RUO
Test Specificity: Recognizes the ∽220kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Content And Storage: 2°C to 8°C
Isotype: IgG1
Antigen:
BRCA1
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Clone:
MS110
Formulation:
In 50mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Immunogen:
recombinant human BRCA1
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽220kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Content And Storage:
2°C to 8°C
Isotype:
IgG1