MilliporeSigma™ anti-p300 Clone: NM-11,

Description

• Specifically detects p300 Clone: NM-11 in Human, Rat samples, and it is validated for Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting p300 is a nuclear phosphoprotein which was originally shown to specifically bind to adenovirus E1A protein
• The binding of p300 to E1A occurs in the conserved region 1 (CR1) of E1A at a site which is close to but non-overlapping with that for the Rb family of proteins including p105 and p107
• p300 has been shown to bind to the extreme N-terminal region requiring the presence of positively charged residues at positions 2 and 3 of E1A via a zinc-finger motif in the C-terminal portion of p300
• While the binding of p300 and Rb to E1A is independent, it has been shown that upon binding, E1A mediates the physical contact between the two proteins
• The p300 protein is actively phosphorylated in both quiescent and proliferating cells however the amount of phosphorylation increases during progression from late G1 intoM phase of the cell cycle
• In addition to the demonstrated binding of p300 to E1A it has also been shown to bind to other molecules including DNA (in a site specific manner) and to components of the TATA binding protein complexes (TBP)
• The TBP complex association is most likely with TFIID and occurs in a protein-protein dependent rather than a DNA dependent manner and, in such a complex, p300 functions as a co-activator of transcription.