Adipolean (gAcrp30) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human gAcrp30/Adipolean
• Anti-Human gAcrp30/Adipolean-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hAdipolean by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean (500-P193G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean
• Western Blot: To detect Human Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P193GBT-1MG will be provided as 2 x 500 μg gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin
• Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein
• Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance
• Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs)
• The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2
• AdipoR2 is predominantly expressed in the liver.