MilliporeSigma™ anti-Rad51 (Ab-1), Polyclonal

Description

• Specifically detects Rad51 Clone: in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Immunoprecipitation The bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair
• The human RAD51 protein is the homologue of these two proteins
• While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA
• Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus
• Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature
• This focal distribution may be blocked by inhibitors of transcription
• RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change
• RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.