MilliporeSigma™ anti-NF-κB (p65, RelA) (Ab-2), Polyclonal

Description

• Specifically detects NF-κB (p65, RelA) Clone: in Human samples, and it is validated for ELISA, Gel Supershift, Immunoprecipitation, Immuno Blotting NFκB (Nuclear factor,kappa;B) is a transcription factor that plays crucial roles in gene regulation including those involved in immune and inflammatory responses
• Active NFκB is most commonly composed of the heterodimer DNA binding subunits p50 and p65 (RelA)
• These subunits are members of a multigene family called the rel family
• In addition to p50 and RelA, three other proteins are implicated in dimer formation leading to transcriptional activation, p52, c- rel and rel B
• Inhibitors to this process exist including another member of the rel family IκBα which binds to c- rel , p65 and relB preventing DNA binding by their homo- or heterodimers
• It has recently been shown that NFκB plays a role in the apoptotic process - when RelA, the gene that codes for an NFκB subunit, was knocked out, this lead to the death of mouse embryos through apoptosis of the liver cells
• Similarly, it has been shown in a wide range of cells that NFκB which has been inactivated by IκBα were more sensitive to TNF-α-induced apoptosis than control cells
• Evidence exists for NFκB playing both anti- and pro-apoptotic roles - it has been suggested that different combinations of subunits may result in different outcomes in terms of cell survival.