CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (218), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Human RANKL/OPGL/TNFSF11/CD254
• This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation
• This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response
• T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss
• This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis
• Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts
• The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy
• Two alternatively spliced transcript variants have been found.