Kanamycin Polyclonal Antibody, Invitrogen™

Description

• PA1-75225 specifically detects Kanamycin
• PA1-75225 has been successfully used in ELISA applications
• The PA1-75225 immunogen is Kanamycin-BTG
• Kanamycin is only found in individuals that have used or taken this drug
• It is an antibiotic complex produced by Streptomyces kanamyceticus from Japanese soil
• Comprises 3 components: kanamycin A, the major component, and kanamycins B and C, the minor components
• [PubChem]Aminoglycosides like kanamycin &quoteirreversibly&quote bind to specific 30S-subunit proteins and 16S rRNA
• Specifically Kanamycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12
• This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit
• This region interacts with the wobble base in the anticodon of tRNA
• This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes
• [National Center for Biotechnology Information
• PubChem Compound Database; CID=6032].