Invitrogen™ MEGAscript™ T7 Transcription Kit

Description

• The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used
• The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase
• Using the MEGAscript T7 Kit: The MEGAscript T7 Kit contains in vitro transcription reaction components and a control template
• The kit will yield a total of 3 to 5mg of RNA (approximately 100μg of RNA or more per reaction) from the control template supplied with the kit
• This corresponds to 400 to 650 moles of RNA for each mole of template
• Smaller templates typically yield a lower mass and a higher molar yield of product
• Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions
• Applications: The MEGAscript T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins
• In large-scale transcription reactions, the concentration of all four nucleotides is high, well above the K m for the enzyme
• The MEGAscript T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions
• The kit is not recommended for synthesis of high specific activity probes
• Accessory products: MessageAmp™ aRNA amplification kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis
• To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr
• No
• AM1908) and the MEGAclear-96 Kit (Mfr.
• No
• AM1909)
• RNA Yield: 100 μg