Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Description

• Ambion mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15 - 35μg of 7-methyl guanosine capped RNA from 1μg of template DNA
• Each kit includes sufficient reagents for 25 reactions
• High yields of capped RNA—up to 39μg of RNA per reaction Extra convenience—cap analog-nucleotide mix provided Simplified reaction format Synthesized transcripts ideal for microinjection, in vitro translation, or transfection in a 20μL reaction during a 2 hour incubation, mMESSAGE mMACHINE High Yield Capped RNA Transcription will yield up to 35μg of capped RNA ™ 10–50 times yield obtained with conventional in vitro transcription reactions Ratio of cap analog to GTP has been optimized to allow compromise between yield (15 - 35μg) and proportion of transcripts that are capped (80%) Contain a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation Optimized for use with polymerases included in kit Contains 57U of cap analog