AM1348
Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1348-Each-of-1 | In Stock | ₹ 53,902.80 |
AM1348 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,902.80
GST (18%): ₹ 9,702.504
Total Price: ₹ 63,605.304
Content And Storage
T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format
Kit
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line
Ambion™, mMESSAGE mMACHINE™
Quantity
25 Reactions
Shipping Condition
Dry Ice
Starting Material
PCR Products, Linearized DNA plasmid
Final Product Type
Synthetic mRNA, mRNA
Sample Type
mRNA, Synthetic mRNA
No. of Reactions
25 Reactions
Product Type
T3 In Vitro Transcription kit
Promoter
T3
Yield
20-30 μg
Related Products
Description
- Ambion mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15 - 35μg of 7-methyl guanosine capped RNA from 1μg of template DNA
- Each kit includes sufficient reagents for 25 reactions
- High yields of capped RNA—up to 39μg of RNA per reaction Extra convenience—cap analog-nucleotide mix provided Simplified reaction format Synthesized transcripts ideal for microinjection, in vitro translation, or transfection in a 20μL reaction during a 2 hour incubation, mMESSAGE mMACHINE High Yield Capped RNA Transcription will yield up to 35μg of capped RNA ™ 10–50 times yield obtained with conventional in vitro transcription reactions Ratio of cap analog to GTP has been optimized to allow compromise between yield (15 - 35μg) and proportion of transcripts that are capped (80%) Contain a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation Optimized for use with polymerases included in kit Contains 57U of cap analog
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Content And Storage: T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, mMESSAGE mMACHINE™
Quantity: 25 Reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
No. of Reactions: 25 Reactions
Product Type: T3 In Vitro Transcription kit
Promoter: T3
Yield: 20-30 μg
Content And Storage:
T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, mMESSAGE mMACHINE™
Quantity:
25 Reactions
Shipping Condition:
Dry Ice
Starting Material:
PCR Products, Linearized DNA plasmid
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
No. of Reactions:
25 Reactions
Product Type:
T3 In Vitro Transcription kit
Promoter:
T3
Yield:
20-30 μg
Content And Storage: E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Control DNA Template should be stored at -20°C.Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): miRNA analysis
Product Line: Ambion™
Quantity: __
Shipping Condition: Dry Ice
Starting Material: RNA (In Vitro Transcribed)
Final Product Type: In Vitro Transcribed RNA (Polyadenylated)
Sample Type: __
No. of Reactions: 25 Reactions
Product Type: __
Promoter: __
Yield: __
Content And Storage:
E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Control DNA Template should be stored at -20°C.Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
miRNA analysis
Product Line:
Ambion™
Quantity:
__
Shipping Condition:
Dry Ice
Starting Material:
RNA (In Vitro Transcribed)
Final Product Type:
In Vitro Transcribed RNA (Polyadenylated)
Sample Type:
__
No. of Reactions:
25 Reactions
Product Type:
__
Promoter:
__
Yield:
__
Content And Storage: __
Format: __
For Use With (Application): __
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Yield:
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Yield:
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