AMB13345
Invitrogen™ MEGAscript™ T7 Transcription Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AMB13345-Each-of-1 | In Stock | ₹ 1,27,655.52 |
AMB13345 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,27,655.52
GST (18%): ₹ 22,977.994
Total Price: ₹ 1,50,633.514
Content And Storage
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format
Kit
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line
Ambion™, MEGAscript™
Quantity
200 reactions
Shipping Condition
Dry Ice
Starting Material
PCR Products, Linearized DNA plasmid
Final Product Type
Synthetic mRNA, mRNA
Sample Type
mRNA, Synthetic mRNA
No. of Reactions
200 Reactions
Product Type
T7 In Vitro Transcription kit
Promoter
T7
Yield
100 μg
Related Products
Description
- The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used
- The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase
- Using the MEGAscript T7 Kit: The MEGAscript T7 Kit contains in vitro transcription reaction components and a control template
- The kit will yield a total of 3 to 5mg of RNA (approximately 100μg of RNA or more per reaction) from the control template supplied with the kit
- This corresponds to 400 to 650 moles of RNA for each mole of template
- Smaller templates typically yield a lower mass and a higher molar yield of product
- Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions
- Applications: The MEGAscript T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins
- In large-scale transcription reactions, the concentration of all four nucleotides is high, well above the K m for the enzyme
- The MEGAscript T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions
- The kit is not recommended for synthesis of high specific activity probes
- Accessory products: MessageAmp™ aRNA amplification kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis
- To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr
- No
- AM1908) and the MEGAclear-96 Kit (Mfr.
- No
- AM1909)
- RNA Yield: 100 μg
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Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, MEGAscript™
Quantity: 200 reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
No. of Reactions: 200 Reactions
Product Type: T7 In Vitro Transcription kit
Promoter: T7
Yield: 100 μg
Content And Storage:
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, MEGAscript™
Quantity:
200 reactions
Shipping Condition:
Dry Ice
Starting Material:
PCR Products, Linearized DNA plasmid
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
No. of Reactions:
200 Reactions
Product Type:
T7 In Vitro Transcription kit
Promoter:
T7
Yield:
100 μg
Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄ARCA Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution,E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Formaldehyde Load Dye are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, mMESSAGE mMACHINE™
Quantity: 50 reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Final Product Type: ARCA Capped Synthetic mRNA, mRNA, Synthetic mRNA
Sample Type: mRNA, Synthetic mRNA
No. of Reactions: 50 Reactions
Product Type: T7 In Vitro Transcription kit
Promoter: T7
Yield: 20-30 μg
Content And Storage:
T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄ARCA Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution,E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Formaldehyde Load Dye are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, mMESSAGE mMACHINE™
Quantity:
50 reactions
Shipping Condition:
Dry Ice
Starting Material:
PCR Products, Linearized DNA plasmid
Final Product Type:
ARCA Capped Synthetic mRNA, mRNA, Synthetic mRNA
Sample Type:
mRNA, Synthetic mRNA
No. of Reactions:
50 Reactions
Product Type:
T7 In Vitro Transcription kit
Promoter:
T7
Yield:
20-30 μg
Content And Storage: Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature. Nuclease-free water may be stored at any temperature.
Format: 96-well Plate
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: MagMAX™
Quantity: 5 x 96 Preps
Shipping Condition: __
Starting Material: __
Final Product Type: Viral DNA⁄RNA
Sample Type: Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
No. of Reactions: 480
Product Type: __
Promoter: __
Yield: 5 ng
Content And Storage:
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature. Nuclease-free water may be stored at any temperature.
Format:
96-well Plate
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
MagMAX™
Quantity:
5 x 96 Preps
Shipping Condition:
__
Starting Material:
__
Final Product Type:
Viral DNA⁄RNA
Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
No. of Reactions:
480
Product Type:
__
Promoter:
__
Yield:
5 ng
Content And Storage: -20°C
Format: __
For Use With (Application): __
Product Line: __
Quantity: 500 μg
Shipping Condition: __
Starting Material: __
Final Product Type: __
Sample Type: __
No. of Reactions: __
Product Type: Antibody
Promoter: __
Yield: __
Content And Storage:
-20°C
Format:
__
For Use With (Application):
__
Product Line:
__
Quantity:
500 μg
Shipping Condition:
__
Starting Material:
__
Final Product Type:
__
Sample Type:
__
No. of Reactions:
__
Product Type:
Antibody
Promoter:
__
Yield:
__