AM1344
Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1344-Each-of-1 | In Stock | ₹ 52,704.96 |
AM1344 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,704.96
GST (18%): ₹ 9,486.893
Total Price: ₹ 62,191.853
Content And Storage
T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format
Kit
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line
Ambion™, mMESSAGE mMACHINE™
Quantity
25 Reactions
Shipping Condition
Dry Ice
Starting Material
PCR Products, Linearized DNA plasmid
Final Product Type
Synthetic mRNA, mRNA
Sample Type
mRNA, Synthetic mRNA
No. of Reactions
25 Reactions
Product Type
T7 In Vitro Transcription kit
Promoter
T7
Yield
20-30 μg
Related Products
Description
- mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA
- Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end
- RNA Yield: 20-30μg Include cap analog [m 7 G(5′)ppp(5′)G] in ultra high-yield transcription reaction Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix
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Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, mMESSAGE mMACHINE™
Quantity: 25 Reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
No. of Reactions: 25 Reactions
Product Type: T7 In Vitro Transcription kit
Promoter: T7
Yield: 20-30 μg
Content And Storage:
T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, mMESSAGE mMACHINE™
Quantity:
25 Reactions
Shipping Condition:
Dry Ice
Starting Material:
PCR Products, Linearized DNA plasmid
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
No. of Reactions:
25 Reactions
Product Type:
T7 In Vitro Transcription kit
Promoter:
T7
Yield:
20-30 μg
Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄ARCA Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution,E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Formaldehyde Load Dye are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, mMESSAGE mMACHINE™
Quantity: 10 Reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Final Product Type: Synthetic mRNA, mRNA, ARCA Capped mRNA
Sample Type: mRNA, Synthetic mRNA
No. of Reactions: 10 Reactions
Product Type: T7 In Vitro Transcription kit
Promoter: T7
Yield: 20-30 μg
Content And Storage:
T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄ARCA Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution,E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Formaldehyde Load Dye are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, mMESSAGE mMACHINE™
Quantity:
10 Reactions
Shipping Condition:
Dry Ice
Starting Material:
PCR Products, Linearized DNA plasmid
Final Product Type:
Synthetic mRNA, mRNA, ARCA Capped mRNA
Sample Type:
mRNA, Synthetic mRNA
No. of Reactions:
10 Reactions
Product Type:
T7 In Vitro Transcription kit
Promoter:
T7
Yield:
20-30 μg
Content And Storage: T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, mMESSAGE mMACHINE™
Quantity: 25 Reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
No. of Reactions: 25 Reactions
Product Type: T3 In Vitro Transcription kit
Promoter: T3
Yield: 20-30 μg
Content And Storage:
T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, mMESSAGE mMACHINE™
Quantity:
25 Reactions
Shipping Condition:
Dry Ice
Starting Material:
PCR Products, Linearized DNA plasmid
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
No. of Reactions:
25 Reactions
Product Type:
T3 In Vitro Transcription kit
Promoter:
T3
Yield:
20-30 μg
Content And Storage: E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Control DNA Template should be stored at -20°C.Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): miRNA analysis
Product Line: Ambion™
Quantity: __
Shipping Condition: Dry Ice
Starting Material: RNA (In Vitro Transcribed)
Final Product Type: In Vitro Transcribed RNA (Polyadenylated)
Sample Type: __
No. of Reactions: 25 Reactions
Product Type: __
Promoter: __
Yield: __
Content And Storage:
E-PAP, 5XE-PAP Buffer, ATP Solution, MnCl2, and Control DNA Template should be stored at -20°C.Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
miRNA analysis
Product Line:
Ambion™
Quantity:
__
Shipping Condition:
Dry Ice
Starting Material:
RNA (In Vitro Transcribed)
Final Product Type:
In Vitro Transcribed RNA (Polyadenylated)
Sample Type:
__
No. of Reactions:
25 Reactions
Product Type:
__
Promoter:
__
Yield:
__