AM1908
Invitrogen™ MEGAclear™ Transcription Clean-Up Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1908-Each-of-1 | In Stock | ₹ 15,229.68 |
AM1908 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,229.68
GST (18%): ₹ 2,741.342
Total Price: ₹ 17,971.022
Content And Storage
Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Format
Tube
Purification Target
mRNA, Synthetic mRNA
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
Ambion™, MEGAclear™
Quantity
20 Preps
Shipping Condition
Room Temperature
Yield
500 μg
Final Product Type
Purified Synthetic mRNA, Purified mRNA
Isolation Technology
Silica Spin Column
Sample Type
mRNA, Synthetic mRNA
For Use With (Equipment)
Vacuum Manifold, Centrifuge
No. of Reactions
20 Preps
Product Type
mRNA purification kit, Purification kit
Purification Time
30 min
Starting Material Volume
1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Related Products
Description
- MEGAclear Kit is designed for quick cleanup of any large-scale transcription reaction
- MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components
- Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components High recovery from 1ng to 500μg of input Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections Quick protocol—purify RNA in less than 30 minutes Using the MEGAclear Kit: Protocol is quick and easy
- Transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is ready for any application requiring highly pure synthetic or in vitro-transcribed RNA
- For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
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Content And Storage: Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Format: Tube
Purification Target: mRNA, Synthetic mRNA
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: Ambion™, MEGAclear™
Quantity: 20 Preps
Shipping Condition: Room Temperature
Yield: 500 μg
Final Product Type: Purified Synthetic mRNA, Purified mRNA
Isolation Technology: Silica Spin Column
Sample Type: mRNA, Synthetic mRNA
For Use With (Equipment): Vacuum Manifold, Centrifuge
No. of Reactions: 20 Preps
Product Type: mRNA purification kit, Purification kit
Purification Time: 30 min
Starting Material Volume: 1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Content And Storage:
Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Format:
Tube
Purification Target:
mRNA, Synthetic mRNA
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
Ambion™, MEGAclear™
Quantity:
20 Preps
Shipping Condition:
Room Temperature
Yield:
500 μg
Final Product Type:
Purified Synthetic mRNA, Purified mRNA
Isolation Technology:
Silica Spin Column
Sample Type:
mRNA, Synthetic mRNA
For Use With (Equipment):
Vacuum Manifold, Centrifuge
No. of Reactions:
20 Preps
Product Type:
mRNA purification kit, Purification kit
Purification Time:
30 min
Starting Material Volume:
1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Content And Storage: __
Format: __
Purification Target: __
For Use With (Application): __
High-throughput Compatibility: __
Product Line: __
Quantity: __
Shipping Condition: __
Yield: __
Final Product Type: __
Isolation Technology: __
Sample Type: __
For Use With (Equipment): __
No. of Reactions: __
Product Type: __
Purification Time: __
Starting Material Volume: __
Content And Storage:
__
Format:
__
Purification Target:
__
For Use With (Application):
__
High-throughput Compatibility:
__
Product Line:
__
Quantity:
__
Shipping Condition:
__
Yield:
__
Final Product Type:
__
Isolation Technology:
__
Sample Type:
__
For Use With (Equipment):
__
No. of Reactions:
__
Product Type:
__
Purification Time:
__
Starting Material Volume:
__
Content And Storage: • 8 ml Binding Solution (store at 4°C)• 20 ml Wash Solution Concentrate (store at 4°C)• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)• 1 MEGAclear™-96 Filter Plate (store at room temperature)• 1 Collection Plate (store at room temperature)• 2 Adhesive Plate Sealers (store at room temperature)
Format: 96-well Plate
Purification Target: mRNA, Synthetic mRNA
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
High-throughput Compatibility: High-throughput Compatible
Product Line: Ambion™, MEGAclear™
Quantity: 96 Preps
Shipping Condition: Room Temperature
Yield: 100 μg
Final Product Type: Purified Synthetic mRNA, Purified mRNA
Isolation Technology: Silica Spin Column
Sample Type: mRNA, Synthetic mRNA
For Use With (Equipment): Vacuum Manifold, Centrifuge
No. of Reactions: 96 Preps
Product Type: mRNA purification kit, Purification kit
Purification Time: 30 min
Starting Material Volume: 1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Content And Storage:
• 8 ml Binding Solution (store at 4°C)• 20 ml Wash Solution Concentrate (store at 4°C)• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)• 1 MEGAclear™-96 Filter Plate (store at room temperature)• 1 Collection Plate (store at room temperature)• 2 Adhesive Plate Sealers (store at room temperature)
Format:
96-well Plate
Purification Target:
mRNA, Synthetic mRNA
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
High-throughput Compatibility:
High-throughput Compatible
Product Line:
Ambion™, MEGAclear™
Quantity:
96 Preps
Shipping Condition:
Room Temperature
Yield:
100 μg
Final Product Type:
Purified Synthetic mRNA, Purified mRNA
Isolation Technology:
Silica Spin Column
Sample Type:
mRNA, Synthetic mRNA
For Use With (Equipment):
Vacuum Manifold, Centrifuge
No. of Reactions:
96 Preps
Product Type:
mRNA purification kit, Purification kit
Purification Time:
30 min
Starting Material Volume:
1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Content And Storage: • 6 mL Formaldehyde Load Dye; store at -20°C• 60 mL Lysis/Binding Solution; 4°C• 60 mL Water; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 40 mL Wash Solution #1; 4°C• 80 mL Wash Solution #2/3 (concentrated); 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Format: __
Purification Target: __
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: __
Quantity: 50 Preps
Shipping Condition: Room Temperature
Yield: __
Final Product Type: Total RNA
Isolation Technology: Spin Column (Glass Fiber Filter)
Sample Type: Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
For Use With (Equipment): __
No. of Reactions: __
Product Type: __
Purification Time: __
Starting Material Volume: __
Content And Storage:
• 6 mL Formaldehyde Load Dye; store at -20°C• 60 mL Lysis/Binding Solution; 4°C• 60 mL Water; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 40 mL Wash Solution #1; 4°C• 80 mL Wash Solution #2/3 (concentrated); 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Format:
__
Purification Target:
__
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
__
Quantity:
50 Preps
Shipping Condition:
Room Temperature
Yield:
__
Final Product Type:
Total RNA
Isolation Technology:
Spin Column (Glass Fiber Filter)
Sample Type:
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
For Use With (Equipment):
__
No. of Reactions:
__
Product Type:
__
Purification Time:
__
Starting Material Volume:
__