Aminophenyl fluorescein (APF) and hydroxyphenyl fluorescein (HPF, H36004), two reactive oxygen species (ROS) indicators developed by T
Nagano, offer greater specificity and stability than dichlorodihydrofluorescein diacetate (H 2 DCFDA, D399)
APF and HPF show limited non-selective reactivity and relatively high resistance to light-induced oxidation Both of these new fluorescein derivatives are nonfluorescent until they react with hydroxyl radical or peroxynitrite anion APF will also react with hypochlorite anion Upon oxidation, both APF and HPF exhibit bright green fluorescence (excitation/ emission maxima approx
490/515nm), making them compatible with any instrument that can detect fluorescein Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitro-Oxidative Stress, Reactive Oxygen Species Order Info Shipping Condition: Room Temperature