B34950
Molecular Probes™BacLight™ Bacterial Membrane Potential Kit
Manufacturer: Molecular Probes™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | B34950-Each | In Stock | ₹ 25,155.85 |
B34950 - Each
In Stock
Quantity
1
Base Price: ₹ 25,155.85
GST (18%): ₹ 4,528.053
Total Price: ₹ 29,683.903
Content And Storage
Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL).Store in refrigerator (2–8°C) and protect from light.
For Use With (Application)
Membrane Potential Assay
Product Type
Bacterial Membrane Potential Kit
Emission
482⁄497
Product Line
BacLight™
Shipping Condition
Wet Ice
Detection Method
Fluorescence
For Use With (Equipment)
Flow Cytometer
Dye Type
Membrane Potential Probes
Format
Tube(s)
Quantity
1 kit
Related Products
Description
- The Bac Light™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC 2 (3), along with a proton ionophore (CCCP) and premixed buffer
- DiOC 2 (3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red
- The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer
- CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.