H 2 DFFDA is nonfluorescent molecule that readily converts to green-fluorescent form when acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within the cell
This fluorinated analog exhibits improved photostability compared to the chlorinated fluorescein derivatives (DCF, DCFDA)
Ex/Em: approx
492–495/517–527nm Product is air sensitive (stored under dry argon or nitrogen) Product may be dissolved in DMSO, DMF, or ethanol for use Indicator is cell permeant Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein Refer to the Molecular Probes™ Handbook for additional product information
Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitro-Oxidative Stress, Reactive Oxygen Species Order Info Shipping Condition: Room Temperature