Invitrogen™ OxyBURST™ Green H2DCFDA, SE

Description

• OxyBURST™ Green H 2 DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) can be used to prepare oxidation-sensitive conjugates of wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres
• Following conjugation to amines, two acetates of OxyBURST™ Green H 2 DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield dihydrofluorescein conjugate
• OxyBURST™ Green H 2 DCFDA conjugates are nonfluorescent until they are oxidized to corresponding fluorescein derivatives
• Ex/Em: approx
• 492 - 495/517–527nm Product is air sensitive (store under dry argon or nitrogen) Product may be dissolved in DMSO, DMF, or ethanol for use Indicator is cell permeant Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein Refer to the Molecular Probes™ Handbook for additional product information
• Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Nitro-Oxidative Stress, Reactive Oxygen Species Order Info Shipping Condition: Room Temperature