CD4 is an approximately 55kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T cells
In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells
The resulting column eluate is a highly enriched CD4+ cell subset population with minimal depletion of the selected T cells Minimal numbers of B cells, monocytes, or non-selected T cell subsets Recovery of CD3+/4+ cells from all available CD3+/4+ cells ranged between 32% and 53% Purity of recovered cell ranging between 88% and 95% with no detectable CD3+/8+ cells among recovered cells