The resulting column eluate is a highly enriched T cell subset population with minimal contamination of either CD45RO+ or CD4+ cells Less than 2% B cells, monocytes, or non-selected T cell subsets Recovery of CD8+/CD45RO- cells from all available CD8+/CD45RO- cells loaded ranged between 25% and 35% Purity (CD8+/CD45RO-) of recovered cells ranged between 85% and 90%, with <1% CD8+/CD45RO+ cells detectable among all cells recovered