K1096
Invitrogen™ LiveBLAzer™ FRET-B/G Loading Kit with CCF4-AM
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1096-Each-of-1 | In Stock | ₹ 5,37,560.00 |
K1096 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,37,560.00
GST (18%): ₹ 96,760.80
Total Price: ₹ 6,34,320.80
Cell Permeability
Cell-Permeant
Detection Method
Fluorescence
Product Line
LiveBLAzer™
Substrate Properties
Chemical Substrate
Substrate
CCF4-AM
Content And Storage
GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
For Use With (Equipment)
Microplate Reader, Fluorometer, Flow Cytometer
Quantity
1 mg
Substrate Type
Beta-Lactamase Substrate
Type
FRET-B/G Loading Kit
Related Products
Description
- Background: GeneBLAzer™ cell-based assays utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4
- These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol
- Detection of GeneBLAzer™ assays is FRET-based
- Each substrate is labeled with two fluorophores that form an efficient FRET pair
- In the absence of beta-lactamase activity, exciting the coumarin at 409 nm in the intact CCF2 molecule results in FRET to the fluorescein, which emits a green fluorescence signal at 518 nm (Figure 1)
- In the presence of beta-lactamase activity, however, cleavage of CCF2 spatially separates the two dyes and disrupts FRET, so that exciting the coumarin at 409 nm now produces a blue fluorescence signal at 447 nm
- This blue signal can be readily observed under a microscope and can also be detected as an increase in the blue channel readout on fluorescent microplate readers
- The CCF2-AM and CCF4-AM substrates are essential assay components for the GeneBLAzer™ platform
- These substrates are fully compatible with flow cytometry, speeding the time to clone selection
- Ratiometric analysis of the blue and green signals reduces well-to-well variation due to differences in cell numbers and substrate loading, leading to high Z´-factor values and low coefficients of variation (CVs)
- CCF2-AM and CCF4-AM differ by two carbons in the bridge linking the coumarin moiety to the lactam ring
- Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells
- CCF4-AM has better solubility properties (soluble for >24 hours) than CCF2-AM and is thus best suited for screening applications
- In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM
- CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version
- CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps
- Cell lysates are the preferred method for applications using cells that contain a cell wall
- CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.
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Cell Permeability: Cell-Permeant
Detection Method: Fluorescence
Product Line: LiveBLAzer™
Substrate Properties: Chemical Substrate
Substrate: CCF4-AM
Content And Storage: GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
For Use With (Equipment): Microplate Reader, Fluorometer, Flow Cytometer
Quantity: 1 mg
Substrate Type: Beta-Lactamase Substrate
Type: FRET-B/G Loading Kit
Cell Permeability:
Cell-Permeant
Detection Method:
Fluorescence
Product Line:
LiveBLAzer™
Substrate Properties:
Chemical Substrate
Substrate:
CCF4-AM
Content And Storage:
GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
For Use With (Equipment):
Microplate Reader, Fluorometer, Flow Cytometer
Quantity:
1 mg
Substrate Type:
Beta-Lactamase Substrate
Type:
FRET-B/G Loading Kit
Cell Permeability: __
Detection Method: __
Product Line: __
Substrate Properties: __
Substrate: __
Content And Storage: Store the unopened product at -20 to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
For Use With (Equipment): __
Quantity: 100 Tests
Substrate Type: __
Type: __
Cell Permeability:
__
Detection Method:
__
Product Line:
__
Substrate Properties:
__
Substrate:
__
Content And Storage:
Store the unopened product at -20 to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
For Use With (Equipment):
__
Quantity:
100 Tests
Substrate Type:
__
Type:
__
Cell Permeability: __
Detection Method: Fluorescence
Product Line: LyticBLAzer™
Substrate Properties: __
Substrate: LyticBLAzer™-FRET B/G
Content And Storage: LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request.LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request.LyticBLAzer™_h-BODIPY™ FL Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-BODIPY™ FL Substrate (provided lyophilized) should be stored at -20C, and protected from light. LyticBLAzer™_h-BODIPY™ FL Substrate should also bedesiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.LyticBLAzer™-BODIPY™ FL Loading Kits include LyticBLAzer™-BODIPY™ FL substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-BODIPY™ FL substrate is provided lyophilized and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored (at 22°C to 25°C, protected from direct light.)
For Use With (Equipment): Fluorometer, Microplate Reader
Quantity: 15 mL
Substrate Type: __
Type: __
Cell Permeability:
__
Detection Method:
Fluorescence
Product Line:
LyticBLAzer™
Substrate Properties:
__
Substrate:
LyticBLAzer™-FRET B/G
Content And Storage:
LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request.LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request.LyticBLAzer™_h-BODIPY™ FL Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-BODIPY™ FL Substrate (provided lyophilized) should be stored at -20C, and protected from light. LyticBLAzer™_h-BODIPY™ FL Substrate should also bedesiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.LyticBLAzer™-BODIPY™ FL Loading Kits include LyticBLAzer™-BODIPY™ FL substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-BODIPY™ FL substrate is provided lyophilized and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored (at 22°C to 25°C, protected from direct light.)
For Use With (Equipment):
Fluorometer, Microplate Reader
Quantity:
15 mL
Substrate Type:
__
Type:
__
Cell Permeability: __
Detection Method: __
Product Line: __
Substrate Properties: __
Substrate: __
Content And Storage: Store the unopened product at -20 to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
For Use With (Equipment): __
Quantity: 100 Tests
Substrate Type: __
Type: __
Cell Permeability:
__
Detection Method:
__
Product Line:
__
Substrate Properties:
__
Substrate:
__
Content And Storage:
Store the unopened product at -20 to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
For Use With (Equipment):
__
Quantity:
100 Tests
Substrate Type:
__
Type:
__