K1607
Thermo Scientific™ Tango™ ADRA2A-blaU2OS DA Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1607-Each-of-1 | In Stock | ₹ 98,051.76 |
K1607 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 98,051.76
GST (18%): ₹ 17,649.317
Total Price: ₹ 1,15,701.077
Accession Number
NM_000681.2
Cell State
Division-Arrested Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Gene Symbol
ADRA2A
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
U2OS
Druggable Target
GPCR
Assay Entry
Cell-based beta-arrestin recruitment
Content And Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ ADRA2A-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ ADRA2A-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ ADRA2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method
Fluorescence
Population Status
Single Cell Clone Origin
Quantity
8 x 106Cells
Target Entry
ADRA2A
System Type
Tango™
Related Products
Description
- The Tango™ ADRA2A-bla U2OS cells contain the human Adrenergic, Alpha-2A-, Receptor (ADRA2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line
- This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element
- The Tango™ ADRA2A-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands
- In addition, Tango™ ADRA2A-bla U2OS cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary for each product
- Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
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Accession Number: NM_000681.2
Cell State: Division-Arrested Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Gene Symbol: ADRA2A
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: U2OS
Druggable Target: GPCR
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ ADRA2A-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ ADRA2A-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ ADRA2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method: Fluorescence
Population Status: Single Cell Clone Origin
Quantity: 8 x 106Cells
Target Entry: ADRA2A
System Type: Tango™
Accession Number:
NM_000681.2
Cell State:
Division-Arrested Cells
Cytoplasmic Tail:
Tail from AVPR2 GPCR
Gene Symbol:
ADRA2A
Product Line:
Tango™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
U2OS
Druggable Target:
GPCR
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ ADRA2A-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ ADRA2A-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ ADRA2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method:
Fluorescence
Population Status:
Single Cell Clone Origin
Quantity:
8 x 106Cells
Target Entry:
ADRA2A
System Type:
Tango™
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
Gene Symbol: __
Product Line: __
Reporter Gene: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: __
Detection Method: __
Population Status: __
Quantity: __
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
Gene Symbol:
__
Product Line:
__
Reporter Gene:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
__
Detection Method:
__
Population Status:
__
Quantity:
__
Target Entry:
__
System Type:
__
Accession Number: __
Cell State: Dividing Cells
Cytoplasmic Tail: __
Gene Symbol: AKT1
Product Line: LanthaScreen™
Reporter Gene: GFP (EmGFP)
Cell Line: 293E (HEK)
Druggable Target: Signaling Pathway
Assay Entry: Cell-based terbium phospho-specific activity (stable)
Content And Storage: LanthaScreen™ AKT HEK 293E cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method: Fluorescence
Population Status: __
Quantity: 1 Vial
Target Entry: AKT1
System Type: LanthaScreen™
Accession Number:
__
Cell State:
Dividing Cells
Cytoplasmic Tail:
__
Gene Symbol:
AKT1
Product Line:
LanthaScreen™
Reporter Gene:
GFP (EmGFP)
Cell Line:
293E (HEK)
Druggable Target:
Signaling Pathway
Assay Entry:
Cell-based terbium phospho-specific activity (stable)
Content And Storage:
LanthaScreen™ AKT HEK 293E cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method:
Fluorescence
Population Status:
__
Quantity:
1 Vial
Target Entry:
AKT1
System Type:
LanthaScreen™
Accession Number: NM_001480
Cell State: Dividing Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Gene Symbol: GALR1
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: U2OS
Druggable Target: GPCR
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes• Tango™ GALR1-bla U2OS cells• Tango™ GALR1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ GALR1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method: Fluorescence
Population Status: Pool of Stable Cells
Quantity: 8 x 106Cells
Target Entry: GALR1
System Type: Tango™
Accession Number:
NM_001480
Cell State:
Dividing Cells
Cytoplasmic Tail:
Tail from AVPR2 GPCR
Gene Symbol:
GALR1
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
U2OS
Druggable Target:
GPCR
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes• Tango™ GALR1-bla U2OS cells• Tango™ GALR1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ GALR1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method:
Fluorescence
Population Status:
Pool of Stable Cells
Quantity:
8 x 106Cells
Target Entry:
GALR1
System Type:
Tango™