K1509
Thermo Scientific™ Tango™ HRH3-blaU2OS DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1509-Each-of-1 | In Stock | ₹ 1,00,276.32 |
K1509 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,00,276.32
GST (18%): ₹ 18,049.738
Total Price: ₹ 1,18,326.058
Accession Number
NM_007232
Cell State
Division-Arrested Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Gene Symbol
HRH3
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
Tango™
Assay Entry
Cell-based beta-arrestin recruitment
Content And Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ HRH3-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ HRH3-bla U2OS Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method
Fluorescence
Population Status
Pool of Stable Cells
Quantity
1 Plate
Target Entry
HRH3, H3
Cell Line
U2OS
Druggable Target
GPCR
Related Products
Description
- The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line
- This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element
- The Tango™ HRH3-bla U2OS cells and the Tango™ HRH3-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of Methylhistamine
- In addition, Tango™ HRH3-bla U2OS cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Compare Similar Items
Show Difference
Accession Number: NM_007232
Cell State: Division-Arrested Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Gene Symbol: HRH3
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
Type: Division-Arrested Cells Assay Kit
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ HRH3-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ HRH3-bla U2OS Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method: Fluorescence
Population Status: Pool of Stable Cells
Quantity: 1 Plate
Target Entry: HRH3, H3
Cell Line: U2OS
Druggable Target: GPCR
Accession Number:
NM_007232
Cell State:
Division-Arrested Cells
Cytoplasmic Tail:
Tail from AVPR2 GPCR
Gene Symbol:
HRH3
Product Line:
Tango™
Reporter Gene:
BLA (Beta-Lactamase)
Type:
Division-Arrested Cells Assay Kit
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ HRH3-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ HRH3-bla U2OS Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method:
Fluorescence
Population Status:
Pool of Stable Cells
Quantity:
1 Plate
Target Entry:
HRH3, H3
Cell Line:
U2OS
Druggable Target:
GPCR
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
Gene Symbol: __
Product Line: One Shot™
Reporter Gene: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Detection Method: __
Population Status: __
Quantity: 20 reactions
Target Entry: __
Cell Line: __
Druggable Target: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
Gene Symbol:
__
Product Line:
One Shot™
Reporter Gene:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Detection Method:
__
Population Status:
__
Quantity:
20 reactions
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Accession Number: NM_001841
Cell State: Dividing Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Gene Symbol: CNR2
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
Type: U2OS Cell
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ CNR2-bla U2OS cells• Tango™ CNR2-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ CNR2-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method: Fluorescence
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: CNR2
Cell Line: U2OS
Druggable Target: GPCR
Accession Number:
NM_001841
Cell State:
Dividing Cells
Cytoplasmic Tail:
Tail from AVPR2 GPCR
Gene Symbol:
CNR2
Product Line:
Tango™
Reporter Gene:
BLA (Beta-Lactamase)
Type:
U2OS Cell
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ CNR2-bla U2OS cells• Tango™ CNR2-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ CNR2-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method:
Fluorescence
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
CNR2
Cell Line:
U2OS
Druggable Target:
GPCR
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
Gene Symbol: __
Product Line: __
Reporter Gene: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: __
Detection Method: __
Population Status: __
Quantity: __
Target Entry: __
Cell Line: __
Druggable Target: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
Gene Symbol:
__
Product Line:
__
Reporter Gene:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
__
Detection Method:
__
Population Status:
__
Quantity:
__
Target Entry:
__
Cell Line:
__
Druggable Target:
__