Thermo Scientific™ GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1357-Each-of-1 | In Stock | ₹ 98,736.24 |
K1357 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 98,736.24
GST (18%): ₹ 17,772.523
Total Price: ₹ 1,16,508.763
Accession Number
NM_001462
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
FPRL-1
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
CHO-K1
Druggable Target
GPCR
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Assay KitEach system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).• FPRL-1-Gα15 CHO-K1 DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D, 1 mL• 2 protocols• Certificate of AnalysisGeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA cellsEach system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).• FPRL-1-Gα15 CHO-K1 DA cells• 1 protocol• Certificate of AnalysisGeneBLAzer™ FPRL-1-Gα15-NFAT-blaCHO-K1 cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).• FPRL-1-Gα15-NFAT-blaCHO-K1 cells• 1 protocol• Certificate of AnalysisStorage:GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Cells and FPRL-1-Gα15-NFAT-blaCHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.
Gene ID (Entrez)
3269
Population Status
Single Cell Clone Origin
Quantity
1 plate
Target Entry
FPRL-1
System Type
GeneBLAzer™
Related Products
Description
- This cell line is available as dividing cells by requesting a quote for K1189 from dividingcells@invitrogen.com GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA (Division Arrested) cells and FPRL-1-Gα15-NFAT- bla CHO-K1 cells contain the human Formyl Peptide-like 1 receptor (FPRL-1) stably integrated into the GeneBLAzer™ Gα15-NFAT- bla CHO-K1 cell line
- GeneBLAzer™ Gα15-NFAT- bla CHO-K1 cells (Cat
- No
- K1213) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element, in addition to the promiscuous G-protein, Gα15
- Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both FPRL-1-Gα15 CHO-K1 DA cells and FPRL-1-Gα15-NFAT- bla CHO-K1 cells are functionally validated for Z'-factor and EC 50 concentrations of WKYMV(M); (Figure 1)
- In addition, FPRL-1-NFAT- bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available
- The FPRL-1 receptor has been implicated in a variety of inflammation and neurological disorders, including Alzheimer's disease.
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Accession Number: NM_001462
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: FPRL-1
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Assay KitEach system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).• FPRL-1-Gα15 CHO-K1 DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D, 1 mL• 2 protocols• Certificate of AnalysisGeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA cellsEach system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).• FPRL-1-Gα15 CHO-K1 DA cells• 1 protocol• Certificate of AnalysisGeneBLAzer™ FPRL-1-Gα15-NFAT-blaCHO-K1 cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).• FPRL-1-Gα15-NFAT-blaCHO-K1 cells• 1 protocol• Certificate of AnalysisStorage:GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Cells and FPRL-1-Gα15-NFAT-blaCHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.
Gene ID (Entrez): 3269
Population Status: Single Cell Clone Origin
Quantity: 1 plate
Target Entry: FPRL-1
System Type: GeneBLAzer™
Accession Number: NM_004382.2
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: CRHR1
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: __
Gene ID (Entrez): 1394
Population Status: Single Cell Clone Origin
Quantity: 1 plate
Target Entry: CRHR1
System Type: GeneBLAzer™
Accession Number: __
Cell State: __
Detection Method: __
Gene Symbol: __
Product Line: __
Reporter Gene: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: __
Gene ID (Entrez): __
Population Status: __
Quantity: __
Target Entry: __
System Type: __