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K1379

Thermo Scientific™ GeneBLAzer™ CCKBR HEK 293T DA Assay Kit

Manufacturer: Thermo Scientific™

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Pack Size	SKU	Availability	Price
Each of 1	K1379-Each-of-1	In Stock	₹ 1,02,706.00

K1379 - Each of 1

₹ 1,02,706.00

In Stock

Quantity

Base Price: ₹ 1,02,706.00

GST (18%): ₹ 18,487.08

Total Price: ₹ 1,21,193.08

Accession Number

NM_176875.2

Cell State

Division-Arrested Cells

Detection Method

Fluorescence

Gene Symbol

CCKBR

Product Line

GeneBLAzer™

Reporter Gene

BLA (Beta-Lactamase)

Cell Line

293T (HEK)

Druggable Target

GPCR

Assay Entry

Cell-based beta lactamase reporter gene

Content And Storage

GeneBLAzer™ CCKBR HEK 293T DA Assay KitEach system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).• CCKBR HEK 293T DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D, 1 mL• 2 protocols• Certificate of AnalysisGeneBLAzer™ CCKBR HEK 293T DA cellsEach system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).• CCKBR HEK 293T DA cells• 1 protocol• Certificate of AnalysisGeneBLAzer™ CCKBR-NFAT-blaHEK 293T cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).• CCKBR-NFAT-blaHEK 293T cells• 1 protocol• Certificate of AnalysisStorageGeneBLAzer™ CCKBR HEK 293T DA Cells and CCKBR-NFAT-blaHEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.

Gene ID (Entrez)

887

Population Status

Single Cell Clone Origin

Quantity

1 Plate

Target Entry

CCKBR

System Type

GeneBLAzer™

Related Products

Description

This cell line is available as dividing cells by requesting a quote for K1231 from dividingcells@invitrogen.com GeneBLAzer™ CCKBR HEK 293T DA (Division Arrested) cells and CCKBR-NFAT- bla HEK 293T cells contain the human Cholecystokinin (CCKBR) receptor stably integrated into the CellSensor™ NFAT- bla HEK 293T cell line
CellSensor™ NFAT- bla HEK 293T cells (Cat
no.K1179) contain a beta-lactamase reporter gene under control of the nuclear factor of activated T-cells (NFAT) response element
Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
Both CCKBR HEK 293T DA cells and CCKBR-NFAT- bla HEK 293T cells are functionally validated for Z'-factor and EC 50 concentrations of cholecystokinin (CCK), (Figure 1)
In addition, CCKBR-NFAT- bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
Additional testing data using alternate stimuli are also available
There are two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR)
Cholecystokinin (CCK) is widely distributed throughout the CNS and digestive system
Through CCKAR and CCKBR, it is thought to play a role in many digestive processes as well as anxiety, depression, psychosis, cognition and nociception
Studies have shown CCKBR could be involved in anxiety, learning and memory, as well as growth of gastric mucosa.

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