Invitrogen™ CellSensor™ NFAT-blaCHO-K1 Cell Line

Description

• The G protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value
• GPCRs are instrumental in the transmission of a wide range of chemical messages from the extracellular environment to the interior of the cell
• GPCRs are involved in a wide range of disorders, including allergies, cardiovascular dysfunction, depression, obesity, cancer, pain, diabetes, and various central nervous system disorders
• GPCR signaling is mediated by trimeric G-proteins containing α, β, and γ subunits and can be categorized into signaling classes based upon subunit composition
• G alpha, G alpha s, and G alpha i/o proteins mediate intracellular signaling through activation of signaling pathways leading to distinct physiological endpoints
• Activation of G alpha s and G alpha i/o coupled receptors leads to stimulation or inhibition of adenylate cyclase, respectively; activation of G alpha q coupled receptors results in stimulation of phospholipase C
• GPCR signaling through these distinct pathways can be monitored by activation of specific transcriptional response elements placed upstream of a reporter gene
• Invitrogen has developed GeneBLAzer™ Master Cell Lines employing the beta-lactamase reporter system, which can be used to screen for both agonists and antagonists of GPCRs coupling to each of the above signaling pathways
• These cell-based assays make use of a membrane permeant fluorescent substrate (CCF4-AM or CCF2-AM), enabling a ratiometric readout that reduces errors due to sample variability, leads to excellent Z'-factor values, and allows for assay miniaturization
• GeneBLAzer™ Master Cell Lines with either NFAT or CRE response elements are available in Jurkat, CHO-K1, or FreeStyle™ 293F cell backgrounds (six cell lines in total) for the development of assays in suspension or adherent cell format
• Stable cell lines employing an NFAT response element upstream of the beta-lactamase gene are designed for the study of Gq coupled receptors
• Stable cell lines employing the cAMP response element (CRE) upstream of the beta-lactamase gene are designed for the study of G s coupled receptors
• FreeStyle™ 293F cells are HEK 293 cells that have been adapted for growth in suspension in serum-free media
• Alternatively, these cells may be grown in the presence of serum for use in the adherent format
• Academic and non-profit customers, please inquire for special pricing.