Invitrogen™ CellSensor™ ARE-blaHep G2 Cell Line

Description

• The CellSensor™ ARE-bla Hep G2 cell line is responsive to tBHQ and Sulforaphane and can be used for analyzing the Nrf2/antioxidant response signaling pathway using the GeneBLAzer™ Loading Substrates
• Reactive oxygen species (ROS) can damage biological macromolecules and are detrimental to cellular health
• Electrophilic compounds, xenobiotics and antioxidants are sources of reactive oxygen species, creating oxidative stress that can harm cells
• Enzymes are involved in the Phase II detoxification of xenobiotics to reduce cellular stress include glutathione transferases, quinone reductase, epoxide hydrolase, heme oxygenase, UDP-glucuronosyl transferases, and gamma-glutamylcysteine synthetase
• Expression of these genes protects cells from oxidative damage and can prevent mutagenesis and cancer
• Transcription of these enzymes is coordinately regulated through antioxidant response elements (AREs)
• Nrf2 (NF-E2-related factor 2) and Nrf1 are transcription factors that bind to AREs and activate these genes
• The CellSensor™ ARE-bla Hep G2 cell line contains a beta-lactamase reporter gene under control of the Antioxidant Response Element (ARE) stably integrated into Hep G2 cells
• To construct this cell line, the ARE-bla construct was transduced into Hep G2 cells
• Flow cytometry was used to isolate cells responsive to tert-butylhydroquinone (tBHQ)
• This cell line is tested under various experimental conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, and functionally validated for Z'; and EC50 concentrations of tBHQ
• Academic and non-profit customers, please inquire for special pricing.