K1710
Thermo Scientific™ GeneBLAzer™ M1-NFAT-blaJurkat Cells
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1710-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1710 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Accession Number
NM_000738
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
M1
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
Jurkat
Druggable Target
GPCR
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ M1-NFAT-blaJurkat cells• GeneBLAzer™ M1-NFAT-blaJurkat Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ M1-NFAT-blaJurkat cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)
FRET
Population Status
Single Cell Clone Origin
Quantity
5 x 106Cells
Target Entry
M1
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ M1-NFAT- bla Jurkat cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor™ NFAT- bla Jurkat cell line
- CellSensor™ NFAT- bla Jurkat cells (Cat
- No
- K1077) contain a beta-lactamase ( bla ) reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element
- M1-NFAT- bla Jurkat cells are functionally validated for Z'-factor and EC 50 concentrations of carbachol
- In addition, M1-NFAT- bla CHO-K1 cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- In the brain, M1 activation mediates 'slow' neuronal excitability
- Cortical and hippocampal muscarinic receptors are thought to be important in the attentional aspects of cognition
- The predominant receptor subtypes in these brain areas are M1, M3, and M4
- Therefore, M1 is a potential target for cognition, Alzheimer's, dementia, and schizophrenia
- Studies on knock-out mouse models of M1 are also beginning to reveal potential functions of the receptor
- Additional information on the muscarinic receptors can be found in reviews
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
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Accession Number: NM_000738
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: M1
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: Jurkat
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ M1-NFAT-blaJurkat cells• GeneBLAzer™ M1-NFAT-blaJurkat Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ M1-NFAT-blaJurkat cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 5 x 106Cells
Target Entry: M1
System Type: GeneBLAzer™
Accession Number:
NM_000738
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
M1
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
Jurkat
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ M1-NFAT-blaJurkat cells• GeneBLAzer™ M1-NFAT-blaJurkat Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ M1-NFAT-blaJurkat cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
5 x 106Cells
Target Entry:
M1
System Type:
GeneBLAzer™
Accession Number: __
Cell State: __
Detection Method: __
Gene Symbol: __
Product Line: __
Reporter Gene: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: The OriginalPichiaExpression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers.Store vectors and primers at -20°C. Store strains at room temperature.All components are guaranteed stable for 6 months when properly stored.
For Use With (Application): Protein Expression
Population Status: __
Quantity: 1 Kit
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Detection Method:
__
Gene Symbol:
__
Product Line:
__
Reporter Gene:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
The OriginalPichiaExpression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers.Store vectors and primers at -20°C. Store strains at room temperature.All components are guaranteed stable for 6 months when properly stored.
For Use With (Application):
Protein Expression
Population Status:
__
Quantity:
1 Kit
Target Entry:
__
System Type:
__
Accession Number: NM_000524
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: 5HT1A
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ 5HT1A Gα15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: 5HT1A, HTR1A
System Type: GeneBLAzer™
Accession Number:
NM_000524
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
5HT1A
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
CHO-K1
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ 5HT1A Gα15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
5HT1A, HTR1A
System Type:
GeneBLAzer™
Accession Number: NM_005226.2
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: EDG3
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: 293T (HEK)
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: EDG3
System Type: GeneBLAzer™
Accession Number:
NM_005226.2
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
EDG3
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
293T (HEK)
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
EDG3
System Type:
GeneBLAzer™