Molecular Probes™ Qtracker™ 525 Cell Labeling Kit (Trial Size)

Description

• The Qtracker™ 525 Cell Labeling Kit uses a custom targeting peptide to deliver green-fluorescent Qdot™ 525 probe (ex/em 405–485/525 nm) into the cytoplasm of live cells
• Once inside the cells, the Qdot™ 525 probe provides an intense, stable fluorescence that can be traced through several generations and is not transferred to adjacent cells in a population
• This trial-size version offers a convenient introduction to the Qtracker™ cell labeling technology at an affordable price
• Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products
• Features of the Qtracker™ Cell Labeling kits include: Qtracker™ probes allow for continuous illumination, without photobleaching or degradation problems often associated with organic dyes Simple to use—add Qtracker™ labeling solution to cells in serum-containing media, incubate one hour, then detect and track the cells Provide an intense, stable fluorescence that can be traced through several generations Excellent tool for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays The Qtracker™ Cell Labeling kits use a custom targeting peptide to deliver Qdot™ probes into the cytoplasm of live cells
• Since the cytoplasmic delivery mechanism is not mediated by a specific enzyme, no cell-type specificity has been observed
• Maximum delivery is typically accomplished in less than 1 hour
• The Qdot™ probes are compatible with serum-sensitive cells
• The intense fluorescence from the Qdot™ probes is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
• Long-Lasting, Targeted Signal Use of Qtracker™ Cell Labeling kits results in the ability to observe labeled cells with extensive continuous illumination, without photobleaching or degradation problems often associated with organic dyes
• Once inside the cells, the Qdot™ probes are localized to cytoplasmic vesicles and are inherited by daughter cells for at least six generations
• Fluorescence from the Qdot™ probes can be detected for up to a week after delivery in some cell lines
• The long-term cellular retention of the Qdot™ probes results in an ideal tool for studying cell motility, migration, differentiation, morphology, and many other cellular function studies
• Since the Qtracker™ labels do not leak out of intact labeled cells and are not transferred to adjacent cells, the Qtracker™ Cell Labeling kits result in a targeted signal
• Monitor the Signal on Multiple Platforms Qtracker™ reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
• Minimal Impact on Live Cells The cytotoxicity of the materials used in Qtracker™ Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells
• Labeling with Qtracker™ Cell Labeling kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
• Useful in a Variety of Cell Tracing Studies Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker™-labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.