Molecular Probes™ FluoVolt™ Membrane Potential Kit

Description

• The FluoVolt™ Membrane Potential Kit represents the next generation in voltage sensitive fluorescent probe-based kits
• The FluoVolt™ probe brings together the best characteristics of the fast and slow response membrane potential fluorescent probes
• The FluoVolt™ probe responds to changes in membrane potential in sub-milliseconds and displays a high magnitude of response
• Features of the FluoVolt™ Membrane Potential Probe include: Fast—responds to changes in membrane potential within sub-milliseconds High sensitivity—response range is typically 25% per 100 mV Emission/excitation works with standard FITC settings Can be used in imaging or patch clamp applications Changes in membrane potential play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, and cell signaling
• Potentiometric fluorescent probes are important tools for studying these processes and are generally characterized as slow- or fast-response probes
• The Best Characteristics of Slow- and Fast-Response Probes Slow-response probes function by entering depolarized cells, binding to proteins or membranes, and exhibiting enhanced fluorescence
• This membrane translocation event decreases the ability of these reporters to respond to changes in membrane potential and introduces a capacitive load that can affect cell health
• However, these probes display a high magnitude of response; typically in the 1% per mV range
• Fast-response probes are molecules that change their structure in response to the surrounding electric field and detect transient (millisecond) potential changes
• However, when compared to the slow-response probes, the fast-response probes have a magnitude of potential-dependent fluorescence change that is often small (2–10% fluorescence change per 100 mV)
• The FluoVolt™ Membrane Potential Probe displays the best properties of the slow- and fast-response probes
• The FluoVolt™ probe is a fast-response probe with a superior potential-dependent fluorescence response
• The response is fast enough to detect transient (sub-millisecond) potential changes in excitable cells and generates a signal change in excess of 25% per 100 mV
• PowerLoad™ and Background Suppressor Solutions Also Included For easy cell loading, the FluoVolt™ Membrane Potential Kit contains PowerLoad™ Concentrate
• Due to the unique nature of the PowerLoad™ solution, it can be used in the presence of complete culture media, thus reducing the negative effects of replacing media or loading in serum-free media
• Baseline autofluorescence caused by components within growth media can be greatly reduced by the addition of the included Neuro Background Supressor
• This solution has been specifically formulated for use with neuronal cells and will not cause osmotic shock
• Additionally, the Neuro Background Suppressor has been used successfully with many different cell types to efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.