Invitrogen™ Dynabeads™ Co-Immunoprecipitation Kit

Description

• The Dynabeads™ Co-Immunoprecipitation Kit is intended for the coupling of an antibody to the surface of Dynabeads™ M-270 Epoxy beads
• The coupled beads and supplied buffers can then be used for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes
• In addition to epoxy-activated Dynabeads magnetic beads, the kit includes the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes
• Benefits of Dynabeads Co-Immunoprecipitation Kit include: • Convenience —contains optimized buffers and epoxy-activated Dynabeads magnetic beads for efficient co-IP • Stabile —gentle separation technology preserves native protein conformations and large protein complexes • Flexibility —allows coupling of any antibody of your choice • Robustness —covalent antibody coupling to the Dynabeads magnetic beads avoids co-elution of the antibody with the target proteins Efficient and easy coupling With this kit, antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate
• Dynabeads M-270 Epoxy beads exhibit ultra-low background binding and do not require blocking before use
• The supplied buffers are optimized to give consistent and reliable results
• The kit includes eight different binding, washing, and elution buffers, in addition to Dynabeads M-270 Epoxy beads, to enable purification of complex protein structures of any size
• All kit components are analytical grade and compatible with protease and phosphatase inhibitors
• Target specific antibody is required for coupling, but not included
• The protocol includes coupling, sample preparation (detergent and cryolysis methods), co-IP, and elution and is compatible with western blot/silver stain, Coomassie stain, mass spectrometry, and extraction of nucleic acids from isolated DNA/RNA binding protein complexes
• Magnetic bead–based separation offers easy handling Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads
• The kit is designed for and tested with cultured yeast and mammalian cells and is compatible with tissue, insect, bacterial, and other lysates
• Automation-ready for use with KingFisher purification systems The 2.8-μm magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher sample purification systems Commercial supply Our manufacturing sites are ISO 13485–certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility, making them an ideal choice for commercial supply
• If you are in the process of customizing the Dynabeads Co-Immunoprecipitation Kit in a commercial product/service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page Order Info Shipping Condition: Room Temperature