Interleukin-2, human

Description

• General description: IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation.[1][2] It is a type I cytokine.[3] The gene is mapped to human chromosome 4q27.[4] Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.
• Application: IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform.[5] It has been used for the production of tumor-specific cytotoxic T lymphocytes.[6] It has been used for the proliferation of T lymphocytes.[7]
• Biochem/physiol Actions: IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.[1][8] It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children.[9][10] Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome).[11] IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.[3]
• Sequence: MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT
• Physical form: Lyophilized with no additives.
• Reconstitution: Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 1 week. For extended storage, freeze in working aliquots. It may be advisable to centrifuge the vial prior to reconstitution.