InnoCyte Monocyte Cell Migration Assay

Description

• General description: The InnoCyte Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.
• Components: 96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.
• Warning: Toxicity: Irritant (B)
• Principle: The Calbiochem InnoCyte 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.
• Preparation Note: Cell Labeling Mixture: Warm the D-PBS and Calcein-AM Solution to room temperature. Add 35 µl Calcein-AM Solution to 10 ml D-PBS. Mix well. Note: Prepare just before use (see step 7 of the Detailed Protocol below).
• Storage and Stability: Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
• Other Notes: Cravens, P.D. 2007. Scan. J. Immunol.65, 514.Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.Cross, A.K., et al. 1997. Cytokine9, 521.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany