Rapid Cell Proliferation Kit

Description

• General description: Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
• Features and Benefits: Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)
• Components: WST-1, 1-Methoxy PMS, and a user protocol.
• Warning: Toxicity: Multiple Toxicity Values, refer to MSDS (O)
• Specifications: Assay Time: 0.5 - 4 h
• Principle: The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
• Preparation Note: • WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.
• Other Notes: Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.Liu, S.O., et al 1995. Nat. Med.1, 267.Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany