Mitochondria Staining Kit

Description

• General description: In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.
• Application: The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.
• Features and Benefits: Kit offers:A fast, simple, and convenient method for staining and assaying mitochondria intactnessContains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potentialContains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregationThe shift in fluorescence is clearly detectable in both green and red channels Suitable for adherent cells as well as cells in suspension Has been tested with Jurkat, U-937, HeLa, NIH3T3 cellsThe fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry