CS0760

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
1 KIT CS0760-1-KIT In Stock ₹ 52,281.00

CS0760 - 1 KIT

₹ 52,281.00

In Stock

Quantity

1

Base Price: ₹ 52,281.00

GST (18%): ₹ 9,410.58

Total Price: ₹ 61,691.58

Quality Level

200

usage

kit sufficient for 1000 reactions (using 96 multiwell plates) kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitableprotein staining: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

application(s)

cell analysisdetection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

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Description

  • Application: Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.[1][2][3]
  • Features and Benefits: Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactnessContains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potentialContains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation
  • Principle: The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.
  • Analysis Note: The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

SAFETY INFORMATION

Signal Word

Danger

Hazard Statements

H300 + H310

Precautionary Statements

P262 - P264 - P280 - P301 + P310 - P302 + P352 + P310 - P361 + P364

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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