Nuclei Isolation Kit: Nuclei EZ Prep

Description

• General description: The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.
• Application: Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
• Biochem/physiol Actions: The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
• Other Notes: All procedures should be carried out on ice or 2-8°C.