M2154
Medium 199
With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): M199 Medium, TCM 199
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 ML | M2154-500-ML | In Stock | ₹ 5,629.00 |
M2154 - 500 ML
In Stock
Quantity
1
Base Price: ₹ 5,629.00
GST (18%): ₹ 1,013.22
Total Price: ₹ 6,642.22
Quality Level
400
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.0-7.6 (25 °C, 209 g/L)
components
sodium pyruvate: noL-glutamine: noNaHCO3: yesHEPES: nophenol red: yesEarle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
Related Products
Description
- General description: Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
- Application: Medium 199 has been used:to culture rumen epithelial cells from yearling Speckle Park beef heifers[1]as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries[2]as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)[3]
- Reconstitution: Supplement with 0.1 g/L L-glutamine.
SAFETY INFORMATION
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Compare Similar Items
Show Difference
Quality Level: 400
sterility: sterile-filtered
form: liquid
technique(s): cell culture | mammalian: suitable
impurities: endotoxin, tested
pH: 7.0-7.6 (25 °C, 209 g/L)
components: sodium pyruvate: noL-glutamine: noNaHCO3: yesHEPES: nophenol red: yesEarle’s salts (5% CO2): yes
shipped in: ambient
storage temp.: 2-8°C
Quality Level:
400
sterility:
sterile-filtered
form:
liquid
technique(s):
cell culture | mammalian: suitable
impurities:
endotoxin, tested
pH:
7.0-7.6 (25 °C, 209 g/L)
components:
sodium pyruvate: noL-glutamine: noNaHCO3: yesHEPES: nophenol red: yesEarle’s salts (5% CO2): yes
shipped in:
ambient
storage temp.:
2-8°C
Quality Level: __
sterility: __
form: __
technique(s): __
impurities: __
pH: __
components: __
shipped in: __
storage temp.: __
Quality Level:
__
sterility:
__
form:
__
technique(s):
__
impurities:
__
pH:
__
components:
__
shipped in:
__
storage temp.:
__
Quality Level: 200
sterility: __
form: liquid
technique(s): __
impurities: ≤0.0005% Phosphorus (P)
pH: __
components: __
shipped in: __
storage temp.: __
Quality Level:
200
sterility:
__
form:
liquid
technique(s):
__
impurities:
≤0.0005% Phosphorus (P)
pH:
__
components:
__
shipped in:
__
storage temp.:
__
Quality Level: __
sterility: __
form: __
technique(s): __
impurities: __
pH: __
components: __
shipped in: __
storage temp.: __
Quality Level:
__
sterility:
__
form:
__
technique(s):
__
impurities:
__
pH:
__
components:
__
shipped in:
__
storage temp.:
__