Tumor Necrosis Factor-α, human (hTNF-α)

Description

• General description: Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.[1]Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.
• Specificity: Human hTNF-α is effective on human and mouse cells.
• Application: Tumor Necrosis Factor-α (hTNF-α) Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.Has activating and growth stimulating activities on a variety of normal cells.Has antiviral activity on many cell types in vitro.Can cause tumor necrosis of certain tumors in vivo.
• Biochem/physiol Actions: Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.[1]
• Quality: Purity: >95% (SDS-PAGE)Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
• Sequence: Chain Length 157 AA
• Unit Definition: EC50 definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).
• Physical form: Solution, filtered through 0.2 μm pore size membrane.
• Preparation Note: Working concentration: 1 ng/mlFor complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.Storage conditions (working solution): -15 to -25 °CIt is recommended to store the solution in aliquots at -15 to -25 °C.Note: Avoid repeated freezing and thawing.
• Other Notes: For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng