Human Collagen Type IV

Description

• General description: Human collagen type IV is a major structural element predominantly found in the basement membrane of the skin. It is a native triple helix and heterotrimeric protein containing two α1-like and one α2-like genes.[1] Type 4 collagen forms a sheet-like extracellular matrix in multicellular tissues that exists around certain cell types like adipocytes, skeletal muscle cells, heart muscle cells, and smooth muscle cells.[2] Human Collagen IV is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type 4 was purified by using differential salt precipitation. Purity and retention of the native helical structure were controlled by SDS-PAGE and by reaction with anti-collagen type-specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL Product Source: Human placenta, negative for HBsAg and HIV antibodies
• Application: Collagen IV from the human placenta is suitable for use as a coating material for cell culture studies and the formation of collagen gels. Human Collagen Type IV has been used: to prepare collagen gel for the invasion assay to provide a supportive matrix for assessing the invasive capacity of monocytes in a cell culture system[3]as an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slides[4]to coat the microchannels of the P96-O2 plate, and provide a surface for the adhesion and growth of endothelial cells (hMVECs) and renal proximal tubular epithelial cells (hRPTECs) in a co-culture system[5]
• Biochem/physiol Actions: Human collagen type IV is associated with Alport and Goodpasture′s syndrome, as well as several rheumatological and dermatological diseases including, acquired epidermolysis bullosa.[1]
• Physical form: Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.
• Storage and Stability: Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.
• Analysis Note: Retention of native structure was confirmed by ability to form microfibrils.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.