Nidogen-1 (NID1) human

Description

• General description: Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins.[1] Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3.[2] Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats.[3] Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
• Application: This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
• Biochem/physiol Actions: Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV.[4] Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation.[5] Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation.[6] Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.[7]