Description

• General description: Chemical structure: macrolide
• Application: Erythromycin has been used:as a supplement for nutrient broth medium for culturing green fluorescent protein (GFP)- expressing E. coli[1]as a model drug to determine small intestinal (SMI) microtissue viability using the MTT assay{254as an antibiotic to study the treatment strategies of chronic infections[2]
• Biochem/physiol Actions: Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.
• Caution: This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.
• Preparation Note: This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.