Description

• General description: Chemical structure: aminoglycoside
• Application: Neomycin trisulfate salt hydrate has been used: to determine Netrin1- encoding gene (Ntn1) protective effects against aminoglycoside antibiotics in cochlear explants[1]for Escherichia coli antibiotic susceptibility test[2] in bone marrow transplantation of mice[3]
• Biochem/physiol Actions: Neomycin Trisulfateis an aminoglycoside antibiotic, which is produced by Streptomyces containing a minimum of 85% neomycin B.[4]It is used to study the cytotoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. Neomycin trisulfate functions as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. This product is recommended for use in cell culture applications at 5 mL/L.
• Caution: Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.
• Preparation Note: Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.
• Other Notes: Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.