F0291
hBFGF
FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
Manufacturer: Sigma Aldrich
CAS Number: 106096-93-9
Synonym(S): Fibroblast Growth Factor-Basic human, FGF2, bFGF
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 μG | F0291-25-μG | In Stock | ₹ 52,769.40 |
F0291 - 25 μG
In Stock
Quantity
1
Base Price: ₹ 52,769.40
GST (18%): ₹ 9,498.492
Total Price: ₹ 62,267.892
biological source
human
Quality Level
200
recombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μgpkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | Sigma Aldrich Fine Chemicals Biosciences Fibroblast Growth Factor-Basic Heparin Stabilized human FGF-Basic, recombinant, expressed in E. coli, sterile-filtered, aqueous solution, suitable for cell culture | 106096-93-9 | MFCD00081382 | 1ML | Sigma Aldrich Fine Chemicals Biosciences | ₹ 15,931.27 | |
 | Fibroblast Growth Factor-Basic from bovine pituitary | Sigma Aldrich | ₹ 90,786.90 |
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Description
- General description: Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
- Application: Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).[1][2][3]
- Physical form: Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
- Analysis Note: The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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biological source: human
Quality Level: 200
recombinant: expressed in E. coli
Assay: ≥97% (SDS-PAGE)
form: lyophilized powder
potency: ≤-0.6 ng/mL
mol wt: 16.0 kDa
packaging: pkg of 4X25 μgpkg of 25 μg
storage condition: avoid repeated freeze/thaw cycles
technique(s): cell culture | mammalian: suitable
biological source:
human
Quality Level:
200
recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
form:
lyophilized powder
potency:
≤-0.6 ng/mL
mol wt:
16.0 kDa
packaging:
pkg of 4X25 μgpkg of 25 μg
storage condition:
avoid repeated freeze/thaw cycles
technique(s):
cell culture | mammalian: suitable
biological source: __
Quality Level: __
recombinant: __
Assay: __
form: __
potency: __
mol wt: __
packaging: __
storage condition: __
technique(s): __
biological source:
__
Quality Level:
__
recombinant:
__
Assay:
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form:
__
potency:
__
mol wt:
__
packaging:
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storage condition:
__
technique(s):
__
biological source: __
Quality Level: __
recombinant: __
Assay: __
form: __
potency: __
mol wt: __
packaging: __
storage condition: __
technique(s): __
biological source:
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Quality Level:
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recombinant:
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Assay:
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form:
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potency:
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mol wt:
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packaging:
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storage condition:
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technique(s):
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biological source: rabbit
Quality Level: 200
recombinant: __
Assay: __
form: buffered aqueous solution
potency: __
mol wt: antigen 115-125 kDa (doublet)
packaging: __
storage condition: __
technique(s): __
biological source:
rabbit
Quality Level:
200
recombinant:
__
Assay:
__
form:
buffered aqueous solution
potency:
__
mol wt:
antigen 115-125 kDa (doublet)
packaging:
__
storage condition:
__
technique(s):
__