Description

• General description: Glutaraldehyde, known as Glutaric dialdehyde or Pentane-1,5-dial, serves as a versatile reagent with applications spanning various research domains, including cell biology and biochemical research. It finds applications as a protein crosslinker, aids in enzyme immobilization for microscopy, and contributes to histochemistry and cytochemistry processes. As an amine-reactive homobifunctional crosslinker, it acts as a cell fixative before procedures like SDS-PAGE, staining, or electron microscopy, making it a valuable tool for diverse morphological studies.Glutaraldehyde demonstrates efficacy in crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it allows direct coupling of biotin hydrazides to nucleic acids, offering potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. Moreover, in histology studies, Glutaraldehyde is employed for preserving tissue sections and preparing them for detailed examination.
• Application: Glutaraldehyde solution has been used:for fixation of mouse[1] and esophageal cancer cell lines[2]to crosslink amyloid-β protein[3]to crosslink gelatin in 3D scaffold preparation of trachea[4]
• Biochem/physiol Actions: Glutaraldehyde is an effective protein crosslinker and finds application in techniques like enzyme immobilisation microscopy, histochemistry and cytochemistry. It exists in different forms under acidic or neutral conditions.[5] It is toxic to aquatic organisms.[6] Its allergic nature leads to hypersensitivity reactions. Contact of glutaraldehyde vapors in endoscopy contributes to Colitis.[7]
• Features and Benefits: Versatile and adaptable for wide variety of research applicationsReady-made solution reduces the need for preparation time
• Packaging: Not refrigerated en route
• Caution: This grade is not recommended for use as an electron microscopy fixative.
• Preparation Note: Not specially purified.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Disclaimer: “The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.”