SRP3015
Beta NGF human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): beta-Nerve Growth Factor
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 20 μG | SRP3015-20-μG | In Stock | ₹ 34,791.55 |
SRP3015 - 20 μG
In Stock
Quantity
1
Base Price: ₹ 34,791.55
GST (18%): ₹ 6,262.479
Total Price: ₹ 41,054.029
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)≥98% (SDS-PAGE)
form
lyophilized
potency
<1.0 ng/mL ED50
mol wt
13.5 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
UniProt accession no.
P01138
Description
- General description: β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.
- Application: β NGF human has been used in immunohistochemistry.[1]
- Biochem/physiol Actions: β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The functional form of human b-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.
- Sequence: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
- Physical form: Lyophilized with no additives.
- Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: human
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: <1.0 ng/mL ED50
mol wt: 13.5 kDa
packaging: pkg of 20 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
UniProt accession no.: P01138
biological source:
human
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
<1.0 ng/mL ED50
mol wt:
13.5 kDa
packaging:
pkg of 20 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
UniProt accession no.:
P01138
biological source: human
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: 5-10 ng/mL ED50
mol wt: 23.8 kDa
packaging: pkg of 10 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
UniProt accession no.: P12644
biological source:
human
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
5-10 ng/mL ED50
mol wt:
23.8 kDa
packaging:
pkg of 10 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
UniProt accession no.:
P12644
biological source: human
recombinant: expressed in HEK 293 cells
Assay: ≥95% (HPLC)≥95% (SDS-PAGE)
form: lyophilized
potency: 0.03-0.06 μg/mL ED50
mol wt: 26.2 kDa
packaging: pkg of 10 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
UniProt accession no.: P22004
biological source:
human
recombinant:
expressed in HEK 293 cells
Assay:
≥95% (HPLC)≥95% (SDS-PAGE)
form:
lyophilized
potency:
0.03-0.06 μg/mL ED50
mol wt:
26.2 kDa
packaging:
pkg of 10 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
UniProt accession no.:
P22004
biological source: human
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: <1.0 ng/mL ED50
mol wt: 13.5 kDa
packaging: pkg of 20 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
UniProt accession no.: P01138
biological source:
human
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
<1.0 ng/mL ED50
mol wt:
13.5 kDa
packaging:
pkg of 20 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
UniProt accession no.:
P01138