BMP-4 human

Description

• General description: Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β (transforming growth factor β) superfamily of structurally related signaling proteins.[1] Escherichia coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor.
• Application: BMP-4 human has been used for in vitro chondrogenesis in goat adipose tissue-derived mesenchymal stem cells.[2]
• Biochem/physiol Actions: Members of TGF-β (transforming growth factor β) superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 (bone morphogenetic protein 4) is involved in the development and maintenance of bone and cartilage.[3] Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.[4] BMP-4 controls the expression of miRNAs in breast cancer cell lines, thereby regulating the proliferation and migration phenotypes.[5] It is involved in carcinogenesis and exhibits its effect through the SMAD-dependent signaling. In mice, homozygous mutations in BMP4 cause early embryonic lethality.[6]
• Sequence: KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
• Physical form: Lyophilized with no additives.
• Reconstitution: Centrifuge the vial prior to opening.  Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml.  Do not vortex.  This solution can be stored at 2-8° C for up to 1 week.  For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20° C to -80° C.