Description

• General description: This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.
• Application: E. coli Extract Total is suitable: for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants[1] to prepare E. coli liposomes for liposomal content release assay[2][3] for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)[4]
• Biochem/physiol Actions: The E.coli lipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin.[8] The E. coli lipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample.[9] It is also used for preparing supported lipid bilayers.[8]
• Packaging: 30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)